3月18日のUC Davisセミナーの講演要旨

Natural variation in leaf morphology results from mutation of a novel KNOX gene
Seisuke Kimura

Striking diversity in size, arrangement, and complexity of leaves can sometimes be seen in closely related species. One such variation is found between wild tomato species collected by Charles Darwin from the Galapagos Islands. Here we show that a single nucleotide deletion in the promoter of the PETROSELINUM (PTS) gene upregulates the gene product in leaves and is responsible for the natural variation in leaf shape in the Galapagean tomatoes. PTS encodes a novel KNOTTED1-LIKE HOMEOBOX (KNOX) gene that lacks a homeodomain. We also showed that the tomato classical mutant bipinnata (bip), which recapitulates the Pts phenotype, results from the loss of function of a BEL-LIKE HOMEODOMAIN (BELL) gene, BIP. We used BiFC (bimolecular fluorescence complementation) and two hybrid competition assays to show that PTS represses KNOX1 protein interactions with BIP, and subsequent nuclear localization of this transcriptional complex. We suggest that natural variation in leaf shape can be created by using a rheostat like mechanism that alters the KNOX1 protein interaction network specifically during leaf development. This subtle change in interaction between transcription factors leaves essential KNOX1 function in the shoot apical meristem intact and appears to be a facile way to alter leaf morphology during evolution.



ベイエリアセミナーとは
Japanese San Francisco Bay Area Seminar は生物学・医学に関するセミナー開催を中心的な活動として研究者支援を行っている非営利団体です。

 
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